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已发表论文
关于“育目调神”针刺法对自闭症谱系障碍大鼠治疗效果的研究
Authors Jia Q, Wang X , Li Z, Li W, Jia R, Yue Z, Zhu Z, Ma B
Received 29 May 2025
Accepted for publication 18 September 2025
Published 25 September 2025 Volume 2025:21 Pages 2195—2210
DOI https://doi.org/10.2147/NDT.S543628
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 2
Editor who approved publication: Dr Yu-Ping Ning
Qianfang Jia,1– 4,* Xiaofang Wang,4,* Zihan Li,5 Wenjing Li,1 Ru Jia,1 Zhixia Yue,1 Zijian Zhu,6 Bingxia Ma7
1Department of Children Rehabilitation, First Affiliated Hospital of Xinxiang Medical University, Xinxiang, People’s Republic of China; 2Xinxiang Key Laboratory of Autism Disease Mechanism Research, Xinxiang, People’s Republic of China; 3Xinxiang Autism Integration Education Engineering and Technology Research Center, Xinxiang, People’s Republic of China; 4Hebei University of Chinese Medicine, Shijiazhuang, People’s Republic of China; 5First Clinical College of Xinxiang Medical University, Xinxiang, People’s Republic of China; 6The First Affiliated Hospital of Xinxiang Medical University, School of Medical Engineering, Xinxiang Medical University, Xinxiang, Henan, People’s Republic of China; 7First Affiliated Hospital of Henan University of Traditional Chinese Medicine, Pediatrics College, Zhengzhou, People’s Republic of China
*These authors contributed equally to this work
Correspondence: Bingxia Ma, Email mbx1963@126.com
Purpose: Acupuncture has been shown to improve symptoms of Autism Spectrum Disorder (ASD), but its underlying mechanisms remain unclear. This study aims to explore the behavioral effects of Yu-Mu-Tiao-Shen Acupuncture on ASD model rats and investigate the potential molecular mechanisms based on SFRP5, β-catenin, and GSK-3β.
Patients and Methods: On gestation day 12.5, SD (Sprague-Dawley) rats were intraperitoneally injected with valproic acid (VPA), and their offspring were considered a reliable ASD rat model. The offspring were randomly assigned to the VPA group or the VPA_acupuncture group (n=7), with a normal group serving as the control. The VPA_acupuncture group underwent Yu-Mu-Tiao-Shen Acupuncture from postnatal days 8 to 28. All rats underwent behavioral testing, including social interaction, open field, and Morris water maze tests. Subsequently, hippocampal and prefrontal cortical tissues were extracted for histological analysis and RNA sequencing to assess gene expression differences across groups, as well as protein expression in hippocampal tissues.
Results: Behavioral tests demonstrated that the Yu-Mu-Tiao-Shen acupuncture technique improved spontaneous activity, abnormal social interaction, and alleviated learning and memory impairments in autistic rat models. In the VPA_acupuncture group, total travel distance, average speed, social interaction time, time spent exploring novel rats, and the number of platform crossings were significantly increased (P < 0.05). Transmission electron microscopy revealed abnormal synaptic and mitochondrial structures in the hippocampal tissue of the VPA group. In contrast, the SD and VPA_acupuncture groups showed relatively intact pre- and post-synaptic membrane structures and normal mitochondrial morphology. Differentially expressed gene (DEG) analysis revealed 111 significantly altered genes in the hippocampus and 282 in the prefrontal cortex between the VPA_acupuncture and VPA groups. Furthermore, in the VPA_acupuncture group, the hippocampal expression levels of SFRP5, β-catenin, and GSK-3β proteins were significantly reduced compared to the model group (P < 0.05).
Conclusion: Yu-Mu-Tiao-Shen Acupuncture can regulate the expression of relevant proteins, modulating synaptic plasticity in hippocampal and prefrontal neurons, which effectively improves motor, social cognitive, and spatial memory abilities in ASD rats. This study provides valuable insights for further exploration of ASD treatments.
Keywords: Yu-Mu points, sishencong points, acupuncture, ASD, autism spectrum disorder, behavioral experiments
1Department of Children Rehabilitation, First Affiliated Hospital of Xinxiang Medical University, Xinxiang, People’s Republic of China; 2Xinxiang Key Laboratory of Autism Disease Mechanism Research, Xinxiang, People’s Republic of China; 3Xinxiang Autism Integration Education Engineering and Technology Research Center, Xinxiang, People’s Republic of China; 4Hebei University of Chinese Medicine, Shijiazhuang, People’s Republic of China; 5First Clinical College of Xinxiang Medical University, Xinxiang, People’s Republic of China; 6The First Affiliated Hospital of Xinxiang Medical University, School of Medical Engineering, Xinxiang Medical University, Xinxiang, Henan, People’s Republic of China; 7First Affiliated Hospital of Henan University of Traditional Chinese Medicine, Pediatrics College, Zhengzhou, People’s Republic of China
*These authors contributed equally to this work
Correspondence: Bingxia Ma, Email mbx1963@126.com
Purpose: Acupuncture has been shown to improve symptoms of Autism Spectrum Disorder (ASD), but its underlying mechanisms remain unclear. This study aims to explore the behavioral effects of Yu-Mu-Tiao-Shen Acupuncture on ASD model rats and investigate the potential molecular mechanisms based on SFRP5, β-catenin, and GSK-3β.
Patients and Methods: On gestation day 12.5, SD (Sprague-Dawley) rats were intraperitoneally injected with valproic acid (VPA), and their offspring were considered a reliable ASD rat model. The offspring were randomly assigned to the VPA group or the VPA_acupuncture group (n=7), with a normal group serving as the control. The VPA_acupuncture group underwent Yu-Mu-Tiao-Shen Acupuncture from postnatal days 8 to 28. All rats underwent behavioral testing, including social interaction, open field, and Morris water maze tests. Subsequently, hippocampal and prefrontal cortical tissues were extracted for histological analysis and RNA sequencing to assess gene expression differences across groups, as well as protein expression in hippocampal tissues.
Results: Behavioral tests demonstrated that the Yu-Mu-Tiao-Shen acupuncture technique improved spontaneous activity, abnormal social interaction, and alleviated learning and memory impairments in autistic rat models. In the VPA_acupuncture group, total travel distance, average speed, social interaction time, time spent exploring novel rats, and the number of platform crossings were significantly increased (P < 0.05). Transmission electron microscopy revealed abnormal synaptic and mitochondrial structures in the hippocampal tissue of the VPA group. In contrast, the SD and VPA_acupuncture groups showed relatively intact pre- and post-synaptic membrane structures and normal mitochondrial morphology. Differentially expressed gene (DEG) analysis revealed 111 significantly altered genes in the hippocampus and 282 in the prefrontal cortex between the VPA_acupuncture and VPA groups. Furthermore, in the VPA_acupuncture group, the hippocampal expression levels of SFRP5, β-catenin, and GSK-3β proteins were significantly reduced compared to the model group (P < 0.05).
Conclusion: Yu-Mu-Tiao-Shen Acupuncture can regulate the expression of relevant proteins, modulating synaptic plasticity in hippocampal and prefrontal neurons, which effectively improves motor, social cognitive, and spatial memory abilities in ASD rats. This study provides valuable insights for further exploration of ASD treatments.
Keywords: Yu-Mu points, sishencong points, acupuncture, ASD, autism spectrum disorder, behavioral experiments