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已发表论文
长链非编码 RNA AC114812 通过 miR-181a-5p-SPP1 轴调节牙周膜细胞的炎症反应
Authors Tong T, Zhao F , Tao R, Liu C, Liu B
Received 5 June 2025
Accepted for publication 11 September 2025
Published 19 September 2025 Volume 2025:18 Pages 13039—13053
DOI https://doi.org/10.2147/JIR.S534691
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 2
Editor who approved publication: Dr Renan Dal Fabbro
Tong Tong,1 Fei Zhao,1 Ran Tao,1 Chunyan Liu,2 Bing Liu1
1Department of Periodontal I, School of Stomatology, Hebei Medical University, Shijiazhuang, People’s Republic of China; 2Department of Orthodontics, School of Stomatology, Hebei Medical University, Shijiazhuang, People’s Republic of China
Correspondence: Bing Liu, School of Stomatology, Hebei Medical University, No. 383, Zhongshan East Road, Chang’an District, Shijiazhuang, Hebei, 050017, People’s Republic of China, Email liubing@hebmu.edu.cn
Background: Long noncoding RNAs (lncRNAs) play a significant role in the occurrence and development of periodontitis. We investigate the potential role of the lncRNA AC114812 in the lipopolysaccharide (LPS) induced proliferation, migration and inflammatory response of periodontal ligament cells (PDLCs) via the miR-181a-5p-SPP1 axis.
Methods: Bioinformatics analysis and whole transcriptome sequencing analysis were conducted on the gingival tissues of three pairs of healthy and periodontitis patients to screen out lncRNAs with differential expression in periodontitis and a periodontitis cell model was constructed via stimulation with LPS. The expression levels of the lncRNA AC114812 in tissues and cells were detected via real-time quantitative polymerase chain reaction (qRT-PCR), and the expression localization was detected via fluorescence in situ hybridization (FISH). The role of si-AC114812 in periodontitis was investigated through qRT-PCR, MTT assay and wound healing experiments. Through database screening combined with sequencing results, the competitive endogenous RNA (ceRNA) mechanism of lncRNA AC114812-miR-181a-5p-SPP1 was verified via a dual-luciferase gene reporter assay.
Results: LncRNA AC114812 was highly expressed in periodontitis tissues. Knockdown of lncRNA AC114812 inhibited the expression of inflammatory factors interleukin-1β (IL-1β) and interleukin-6 (IL-6) in PDLCs stimulated by inflammation and improved the proliferation and migration abilities of PDLCs affected by inflammation. FISH assays confirmed that the lncRNA AC114812 was expressed mainly in cytoplasm and may have a sponge effect. The ceRNA mechanism of the lncRNA AC114812-miR-181a-5p-SPP1 was predicted. The dual-luciferase gene reporter assay verified the existence of binding sites among the three genes and their mutual regulatory effects.
Conclusion: By regulating the expression of long non-coding RNA AC114812, the inflammatory response can be alleviated, thereby affecting cell proliferation and migration. The effect may be achieved through the miR-181a-5p-SPP1 axis. These can provide new strategies and intervention targets for the prevention and treatment of periodontal diseases.
Keywords: periodontitis, LncRNA AC114812, inflammatory response
1Department of Periodontal I, School of Stomatology, Hebei Medical University, Shijiazhuang, People’s Republic of China; 2Department of Orthodontics, School of Stomatology, Hebei Medical University, Shijiazhuang, People’s Republic of China
Correspondence: Bing Liu, School of Stomatology, Hebei Medical University, No. 383, Zhongshan East Road, Chang’an District, Shijiazhuang, Hebei, 050017, People’s Republic of China, Email liubing@hebmu.edu.cn
Background: Long noncoding RNAs (lncRNAs) play a significant role in the occurrence and development of periodontitis. We investigate the potential role of the lncRNA AC114812 in the lipopolysaccharide (LPS) induced proliferation, migration and inflammatory response of periodontal ligament cells (PDLCs) via the miR-181a-5p-SPP1 axis.
Methods: Bioinformatics analysis and whole transcriptome sequencing analysis were conducted on the gingival tissues of three pairs of healthy and periodontitis patients to screen out lncRNAs with differential expression in periodontitis and a periodontitis cell model was constructed via stimulation with LPS. The expression levels of the lncRNA AC114812 in tissues and cells were detected via real-time quantitative polymerase chain reaction (qRT-PCR), and the expression localization was detected via fluorescence in situ hybridization (FISH). The role of si-AC114812 in periodontitis was investigated through qRT-PCR, MTT assay and wound healing experiments. Through database screening combined with sequencing results, the competitive endogenous RNA (ceRNA) mechanism of lncRNA AC114812-miR-181a-5p-SPP1 was verified via a dual-luciferase gene reporter assay.
Results: LncRNA AC114812 was highly expressed in periodontitis tissues. Knockdown of lncRNA AC114812 inhibited the expression of inflammatory factors interleukin-1β (IL-1β) and interleukin-6 (IL-6) in PDLCs stimulated by inflammation and improved the proliferation and migration abilities of PDLCs affected by inflammation. FISH assays confirmed that the lncRNA AC114812 was expressed mainly in cytoplasm and may have a sponge effect. The ceRNA mechanism of the lncRNA AC114812-miR-181a-5p-SPP1 was predicted. The dual-luciferase gene reporter assay verified the existence of binding sites among the three genes and their mutual regulatory effects.
Conclusion: By regulating the expression of long non-coding RNA AC114812, the inflammatory response can be alleviated, thereby affecting cell proliferation and migration. The effect may be achieved through the miR-181a-5p-SPP1 axis. These can provide new strategies and intervention targets for the prevention and treatment of periodontal diseases.
Keywords: periodontitis, LncRNA AC114812, inflammatory response