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已发表论文
单核细胞/巨噬细胞相关基因 Ccl9 在急性心肌梗死中的作用分析：基于转录组和单细胞 RNA 测序数据
Authors Yang Q , Luo Z , Cheng X, Wang T, Hu P
Received 15 January 2025
Accepted for publication 26 September 2025
Published 31 October 2025 Volume 2025:18 Pages 15187—15206
DOI https://doi.org/10.2147/JIR.S516092
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 2
Editor who approved publication: Professor Ning Quan
Qiang Yang,1 Ziyue Luo,2 Xinyi Cheng,2 Tianyu Wang,2 Pengfei Hu1
1Department of Cardiology, The Second Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang, 310005, People’s Republic of China; 2Second Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, 310053, People’s Republic of China
Correspondence: Pengfei Hu, Department of Cardiology, The Second Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang, 310005, People’s Republic of China, Tel +86 15267037741, Email 20064012@zcmu.edu.cn
Background: Acute myocardial infarction (AMI) is one of the major causes of sudden cardiac death, resulting in severe complications. Therefore, it is of great significance to find new therapeutic targets.
Methods: AMI datasets, Bulk RNA sequencing (Bulk RNA seq) GSE158157 and GSE23294, scRNA-seq GSE163129 datasets were downloaded from GEO. In this study, GSE163129 was subjected to dimensionality reduction analysis, subpopulation identification, enrichment analysis, and cellular communication analysis. Subsequently, we performed differential expression and immune infiltration analysis on GSE158157 and GSE23294 datasets to obtain seven monocyte/macrophage-related core genes. Finally, the mechanism of Ccl9 in AMI was explored through a series of animal and cellular experiments, which involved the establishment of AMI mouse model and isolation of primary mouse cardiomyocytes.
Results: The t-distributed stochastic neighbor embedding (tSNE) analysis of GSE163129 identified 18 cell clusters, divided into six cell types. Monocytes and neutrophils were highly active. Differential expression analysis of GSE158157 and GSE23294 identified 2362 differentially expressed genes (DEGs). Immune infiltration analysis showed macrophage presence, with notable differences in M2 monocytes and other immune cells between AMI and Sham groups. Nine monocyte-associated hub genes were identified. qRT-PCR validation in AMI mouse models revealed significant upregulation of B2M, SPP1, Ccl4, LGALS3, MRC1, Ccl9, and FN1. Co-culture of primary cardiomyocytes and RAW264.7 cells indicated that low Ccl9 expression promoted M2 macrophage polarization and reduced OGD-induced cardiomyocyte injury.
Conclusion: This study identified seven monocyte/macrophage-related genes in AMI and highlighted Ccl9’s potential role in regulating M2 macrophage polarization and mitigating OGD-induced cardiomyocyte injury in AMI.
Keywords: acute myocardial infarction, Ccl9, monocyte, macrophage, ScRNA-seq
1Department of Cardiology, The Second Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang, 310005, People’s Republic of China; 2Second Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, 310053, People’s Republic of China
Correspondence: Pengfei Hu, Department of Cardiology, The Second Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang, 310005, People’s Republic of China, Tel +86 15267037741, Email 20064012@zcmu.edu.cn
Background: Acute myocardial infarction (AMI) is one of the major causes of sudden cardiac death, resulting in severe complications. Therefore, it is of great significance to find new therapeutic targets.
Methods: AMI datasets, Bulk RNA sequencing (Bulk RNA seq) GSE158157 and GSE23294, scRNA-seq GSE163129 datasets were downloaded from GEO. In this study, GSE163129 was subjected to dimensionality reduction analysis, subpopulation identification, enrichment analysis, and cellular communication analysis. Subsequently, we performed differential expression and immune infiltration analysis on GSE158157 and GSE23294 datasets to obtain seven monocyte/macrophage-related core genes. Finally, the mechanism of Ccl9 in AMI was explored through a series of animal and cellular experiments, which involved the establishment of AMI mouse model and isolation of primary mouse cardiomyocytes.
Results: The t-distributed stochastic neighbor embedding (tSNE) analysis of GSE163129 identified 18 cell clusters, divided into six cell types. Monocytes and neutrophils were highly active. Differential expression analysis of GSE158157 and GSE23294 identified 2362 differentially expressed genes (DEGs). Immune infiltration analysis showed macrophage presence, with notable differences in M2 monocytes and other immune cells between AMI and Sham groups. Nine monocyte-associated hub genes were identified. qRT-PCR validation in AMI mouse models revealed significant upregulation of B2M, SPP1, Ccl4, LGALS3, MRC1, Ccl9, and FN1. Co-culture of primary cardiomyocytes and RAW264.7 cells indicated that low Ccl9 expression promoted M2 macrophage polarization and reduced OGD-induced cardiomyocyte injury.
Conclusion: This study identified seven monocyte/macrophage-related genes in AMI and highlighted Ccl9’s potential role in regulating M2 macrophage polarization and mitigating OGD-induced cardiomyocyte injury in AMI.
Keywords: acute myocardial infarction, Ccl9, monocyte, macrophage, ScRNA-seq