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已发表论文
从下呼吸道标本中分离出的金黄色葡萄球菌小菌落变异株的特征描述
Authors An C , Zou S, Chen S, Chen R, Wu B, Lin Y, Luo C, Yang B
Received 28 July 2025
Accepted for publication 4 November 2025
Published 13 November 2025 Volume 2025:18 Pages 5927—5937
DOI https://doi.org/10.2147/IDR.S556503
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 2
Editor who approved publication: Dr Hazrat Bilal
Chao An,1– 3 Shenghua Zou,4 Shanjian Chen,1– 3 Ruan Chen,5 Baijian Wu,5 Yulan Lin,1– 3 Chenshuo Luo,1– 3 Bin Yang1– 3
1Department of Laboratory Medicine, The First Affiliated Hospital, Fujian Medical University, Fuzhou, 350005, People’s Republic of China; 2Clinical Laboratory Diagnostics, The First Clinical College, Fujian Medical University, Fuzhou, 350005, People’s Republic of China; 3Fujian Key Laboratory of Laboratory Medicine, The First Affiliated Hospital, Fujian Medical University, Fuzhou, 350005, People’s Republic of China; 4Department of Laboratory Medicine, Fuzhou Pulmonary Hospital of Fujian Province, Fuzhou, 350008, People’s Republic of China; 5Department of Orthopaedic Surgery, The First Affiliated Hospital, Fujian Medical University, Fuzhou, 350005, People’s Republic of China
Correspondence: Bin Yang, Department of Laboratory Medicine, The First Affiliated Hospital, Fujian Medical University, No. 20 Chazhong Road, Taijiang District, Fuzhou, Fujian, 350005, People’s Republic of China, Email yangbin2864@163.com Chenshuo Luo, Department of Laboratory Medicine, The First Affiliated Hospital, Fujian Medical University, No. 20 Chazhong Road, Taijiang District, Fuzhou, Fujian, 350005, People’s Republic of China, Email 1017652575@qq.com
Purpose: Epidemiological data on small colony variants (SCVs) of Staphylococcus aureus (S. aureus) in China are lacking. This study aimed to investigate the prevalence and characteristics of S. aureus SCVs in patients with Pseudomonas aeruginosa (P. aeruginosa) pneumonia.
Methods: From October 2024 to September 2025, S. aureus SCVs were collected from lower respiratory tract specimens at two tertiary hospitals in Fuzhou and identified using MALDI-TOF MS. Antibiotic susceptibility testing was performed using a VITEK® 2 Compact System. Genetic diversity and virulence were analyzed using multilocus sequence typing (MLST), staphylococcal protein A (spa) typing, and toxin gene profiling. Biofilm formation was assessed using a microtiter plate assay, and patient characteristics were analyzed using the Hospital Information System.
Results: Thirty-eight S. aureus SCVs (2.1%) were isolated from 1,832 lower respiratory tract specimens collected from patients with P. aeruginosa pneumonia. Compared to normal phenotype strains, SCVs exhibited smaller colonies and reduced hemolysis. Among resistant strains, 20 were methicillin-resistant S. aureus SCVs (MRSA-SCVs), with ST1-t128 (25.0%) being the most prevalent. ST764-t1084 MRSA-SCVs were resistant to penicillin, oxacillin, gentamicin, ciprofloxacin, levofloxacin, moxifloxacin, erythromycin, clindamycin, and tetracycline. Eighteen strains were methicillin-susceptible S. aureus SCVs (MSSA-SCVs), predominantly ST72-t3735 (16.7%). Virulence analysis showed adhesion-related gene carriage rates of 47.4– 100.0% and immune evasion gene carriage rates of 52.6– 73.7%. In addition, most S. aureus SCVs showed strong biofilm production.
Conclusion: This study identified a 2.1% prevalence of S. aureus SCVs (often undetected) in P. aeruginosa pneumonia patients. More than half of patients were methicillin-resistant (MRSA), with strong biofilm-forming capacity and a potential association with prolonged hospitalization. Vigilance is warranted against potential outbreaks of the predominant MRSA-SCV clone ST1-t128, as well as the severe drug resistance observed in ST764-t1084 MRSA-SCVs.
Keywords: Staphylococcus aureus, small colony variants, MLST, antibiotic susceptibility, biofilm
1Department of Laboratory Medicine, The First Affiliated Hospital, Fujian Medical University, Fuzhou, 350005, People’s Republic of China; 2Clinical Laboratory Diagnostics, The First Clinical College, Fujian Medical University, Fuzhou, 350005, People’s Republic of China; 3Fujian Key Laboratory of Laboratory Medicine, The First Affiliated Hospital, Fujian Medical University, Fuzhou, 350005, People’s Republic of China; 4Department of Laboratory Medicine, Fuzhou Pulmonary Hospital of Fujian Province, Fuzhou, 350008, People’s Republic of China; 5Department of Orthopaedic Surgery, The First Affiliated Hospital, Fujian Medical University, Fuzhou, 350005, People’s Republic of China
Correspondence: Bin Yang, Department of Laboratory Medicine, The First Affiliated Hospital, Fujian Medical University, No. 20 Chazhong Road, Taijiang District, Fuzhou, Fujian, 350005, People’s Republic of China, Email yangbin2864@163.com Chenshuo Luo, Department of Laboratory Medicine, The First Affiliated Hospital, Fujian Medical University, No. 20 Chazhong Road, Taijiang District, Fuzhou, Fujian, 350005, People’s Republic of China, Email 1017652575@qq.com
Purpose: Epidemiological data on small colony variants (SCVs) of Staphylococcus aureus (S. aureus) in China are lacking. This study aimed to investigate the prevalence and characteristics of S. aureus SCVs in patients with Pseudomonas aeruginosa (P. aeruginosa) pneumonia.
Methods: From October 2024 to September 2025, S. aureus SCVs were collected from lower respiratory tract specimens at two tertiary hospitals in Fuzhou and identified using MALDI-TOF MS. Antibiotic susceptibility testing was performed using a VITEK® 2 Compact System. Genetic diversity and virulence were analyzed using multilocus sequence typing (MLST), staphylococcal protein A (spa) typing, and toxin gene profiling. Biofilm formation was assessed using a microtiter plate assay, and patient characteristics were analyzed using the Hospital Information System.
Results: Thirty-eight S. aureus SCVs (2.1%) were isolated from 1,832 lower respiratory tract specimens collected from patients with P. aeruginosa pneumonia. Compared to normal phenotype strains, SCVs exhibited smaller colonies and reduced hemolysis. Among resistant strains, 20 were methicillin-resistant S. aureus SCVs (MRSA-SCVs), with ST1-t128 (25.0%) being the most prevalent. ST764-t1084 MRSA-SCVs were resistant to penicillin, oxacillin, gentamicin, ciprofloxacin, levofloxacin, moxifloxacin, erythromycin, clindamycin, and tetracycline. Eighteen strains were methicillin-susceptible S. aureus SCVs (MSSA-SCVs), predominantly ST72-t3735 (16.7%). Virulence analysis showed adhesion-related gene carriage rates of 47.4– 100.0% and immune evasion gene carriage rates of 52.6– 73.7%. In addition, most S. aureus SCVs showed strong biofilm production.
Conclusion: This study identified a 2.1% prevalence of S. aureus SCVs (often undetected) in P. aeruginosa pneumonia patients. More than half of patients were methicillin-resistant (MRSA), with strong biofilm-forming capacity and a potential association with prolonged hospitalization. Vigilance is warranted against potential outbreaks of the predominant MRSA-SCV clone ST1-t128, as well as the severe drug resistance observed in ST764-t1084 MRSA-SCVs.
Keywords: Staphylococcus aureus, small colony variants, MLST, antibiotic susceptibility, biofilm