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已发表论文
层叠抗体嵌入金网：一种有效检测循环肿瘤细胞的早期方法
Authors Qin R, Ma Z, Song C, Huang C, Zhu Z , Yan J, Wang J
Received 9 May 2025
Accepted for publication 17 September 2025
Published 10 November 2025 Volume 2025:20 Pages 13563—13571
DOI https://doi.org/10.2147/IJN.S529248
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 3
Editor who approved publication: Professor Farooq A. Shiekh
Rong Qin,1,2 Zhihao Ma,3 Chenglong Song,3 Chenghong Huang,4 Zhiqiang Zhu,5 Jing Yan,6 Jie Wang3
1Department of Medical Oncology, Jiangsu University Affiliated People’s Hospital, Zhenjiang, People’s Republic of China; 2Zhenjiang Clinical Medical College of Nanjing Medical University, Zhenjiang, People’s Republic of China; 3Institute for Advanced Materials, School of Materials Science and Engineering, Jiangsu University, Zhenjiang, 212013, People’s Republic of China; 4Chongqing university of Science and Technology, Chongqing, 401331, People’s Republic of China; 5Suzhou Chien-Shiung Institute of Technology, Suzhou, 215123, People’s Republic of China; 6Holosensor Medical Ltd., Suzhou, 215000, People’s Republic of China
Correspondence: Jie Wang, Email wangjie@ujs.edu.cn Jing Yan, Email yj@hemosmartmed.com
Introduction: The importance of early cancer diagnosis has been recognized for decades, driving the demand for technological advancements and novel strategies for cancer detection. The conventional detection of circulating tumor cells (CTCs) often relies on size-based separation to distinguish CTCs from other blood cells. However, this method frequently leads to significant cell congestion and poorly recognizable fluorescent images, which inevitably reduces the sensitivity and specificity of CTC detection. Most CTC current separation devices with a cell filtration process have a cell capture efficiency ranging from 50% to 80% in clinical application.
Methods: We constructed a flexible antibody network on the surface of gold-plated iron meshes with a pore size of 20 μm using the layer-by-layer (LbL) assembly technique. These meshes were then used to enrich MCF7 cells and CTCs in 10 clinical blood samples from breast cancer patients.
Results and the Conclusion: This antibody network reduced the effective pore size, thereby improving both capture efficiency and specificity for CTCs. In a cell line separation study, meshes with a trilayered antibody network demonstrated a capture efficiency of 65% compared to 26% for those with a single layer. In tests using clinical samples, the trilayered antibody network achieved 100% accuracy, whereas the single-layer configuration only reached 40%. The multilayered antibody network shows strong potential for enhancing widely used immunosensors.
Keywords: circulating tumor cells, CTCs, immunosensor, layer-by-layer assembly, LbL
1Department of Medical Oncology, Jiangsu University Affiliated People’s Hospital, Zhenjiang, People’s Republic of China; 2Zhenjiang Clinical Medical College of Nanjing Medical University, Zhenjiang, People’s Republic of China; 3Institute for Advanced Materials, School of Materials Science and Engineering, Jiangsu University, Zhenjiang, 212013, People’s Republic of China; 4Chongqing university of Science and Technology, Chongqing, 401331, People’s Republic of China; 5Suzhou Chien-Shiung Institute of Technology, Suzhou, 215123, People’s Republic of China; 6Holosensor Medical Ltd., Suzhou, 215000, People’s Republic of China
Correspondence: Jie Wang, Email wangjie@ujs.edu.cn Jing Yan, Email yj@hemosmartmed.com
Introduction: The importance of early cancer diagnosis has been recognized for decades, driving the demand for technological advancements and novel strategies for cancer detection. The conventional detection of circulating tumor cells (CTCs) often relies on size-based separation to distinguish CTCs from other blood cells. However, this method frequently leads to significant cell congestion and poorly recognizable fluorescent images, which inevitably reduces the sensitivity and specificity of CTC detection. Most CTC current separation devices with a cell filtration process have a cell capture efficiency ranging from 50% to 80% in clinical application.
Methods: We constructed a flexible antibody network on the surface of gold-plated iron meshes with a pore size of 20 μm using the layer-by-layer (LbL) assembly technique. These meshes were then used to enrich MCF7 cells and CTCs in 10 clinical blood samples from breast cancer patients.
Results and the Conclusion: This antibody network reduced the effective pore size, thereby improving both capture efficiency and specificity for CTCs. In a cell line separation study, meshes with a trilayered antibody network demonstrated a capture efficiency of 65% compared to 26% for those with a single layer. In tests using clinical samples, the trilayered antibody network achieved 100% accuracy, whereas the single-layer configuration only reached 40%. The multilayered antibody network shows strong potential for enhancing widely used immunosensors.
Keywords: circulating tumor cells, CTCs, immunosensor, layer-by-layer assembly, LbL