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已发表论文
用于水痘带状疱疹病毒诊断的高活性及稳定性的金@铂纳米酶基比色核酸检测法
Authors Zong M, Liu F, Long L, Liu J, Wu X
Received 3 September 2025
Accepted for publication 5 November 2025
Published 8 November 2025 Volume 2025:20 Pages 13489—13498
DOI https://doi.org/10.2147/IJN.S554033
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 2
Editor who approved publication: Dr Kamakhya Misra
Mingji Zong,1,* Fengshou Liu,1,* Lin Long,2 Jianbo Liu,1 Xiaochun Wu3
1College of Opto-Electronic Engineering, Zaozhuang University, Zaozhuang, People’s Republic of China; 2Zaozhuang Municipal Center for Disease Control and Prevention, Zaozhuang, People’s Republic of China; 3CAS Key Laboratory of Standardization and Measurement for Nanotechnology, National Center for Nanoscience and Technology, Beijing, People’s Republic of China
*These authors contributed equally to this work
Correspondence: Lin Long, Zaozhuang Municipal Center for Disease Control and Prevention, Zaozhuang, People’s Republic of China, Email zzcdcll@163.com Jianbo Liu, College of Opto-electronic Engineering, Zaozhuang University, Zaozhuang, People’s Republic of China, Email linyibm@163.com
Purpose: Currently, DNA is primarily detected using real-time quantitative polymerase chain reaction (qPCR) kits. However, their widespread clinical application is hindered by their high cost and the need for sophisticated instrumentation. Consequently, there is an urgent need to develop simpler DNA screening methods that are both cost-effective and compatible with resource-limited settings, thereby eliminating the need for complex equipment.
Methods: Taking advantage of the DNA-controlled property as well as the ultrahigh peroxidase-like catalytic activity of the Au@Pt nanozymes, we developed a simple and sensitive platform for the colorimetric detection of DNA sequence of varicella-zoster virus.
Results: Visual inspection of chromogenic reactions clearly demonstrated that the target DNA effectively inhibited the peroxidase-like activity of Au@Pt nanozymes. The response ranging from 1 to 1000 ng/mL, combined with a detection limit of 1ng/mL confirms the remarkable sensitivity of DNA-mediated regulation of Au@Pt nanozyme activity. Specificity assessment against other common infectious viruses revealed exceptional selectivity of the Au@Pt nanozyme system for varicella-zoster viruses. Moreover, the robust stability (retaining functionality for four weeks) of Au@Pt nanozymes further enhanced their practical utility.
Conclusion: This novel technology demonstrates high specificity for target DNA and achieved perfect agreement with RT-PCR in clinical validation. Its combination of operational simplicity, requiring no instrumentation, and remarkable stability positions it as a highly promising tool for diagnosing infectious diseases.
Keywords: nanozyme, core/shell nanostructures, nucleic acid detection, varicella-zoster virus, diagnosis
1College of Opto-Electronic Engineering, Zaozhuang University, Zaozhuang, People’s Republic of China; 2Zaozhuang Municipal Center for Disease Control and Prevention, Zaozhuang, People’s Republic of China; 3CAS Key Laboratory of Standardization and Measurement for Nanotechnology, National Center for Nanoscience and Technology, Beijing, People’s Republic of China
*These authors contributed equally to this work
Correspondence: Lin Long, Zaozhuang Municipal Center for Disease Control and Prevention, Zaozhuang, People’s Republic of China, Email zzcdcll@163.com Jianbo Liu, College of Opto-electronic Engineering, Zaozhuang University, Zaozhuang, People’s Republic of China, Email linyibm@163.com
Purpose: Currently, DNA is primarily detected using real-time quantitative polymerase chain reaction (qPCR) kits. However, their widespread clinical application is hindered by their high cost and the need for sophisticated instrumentation. Consequently, there is an urgent need to develop simpler DNA screening methods that are both cost-effective and compatible with resource-limited settings, thereby eliminating the need for complex equipment.
Methods: Taking advantage of the DNA-controlled property as well as the ultrahigh peroxidase-like catalytic activity of the Au@Pt nanozymes, we developed a simple and sensitive platform for the colorimetric detection of DNA sequence of varicella-zoster virus.
Results: Visual inspection of chromogenic reactions clearly demonstrated that the target DNA effectively inhibited the peroxidase-like activity of Au@Pt nanozymes. The response ranging from 1 to 1000 ng/mL, combined with a detection limit of 1ng/mL confirms the remarkable sensitivity of DNA-mediated regulation of Au@Pt nanozyme activity. Specificity assessment against other common infectious viruses revealed exceptional selectivity of the Au@Pt nanozyme system for varicella-zoster viruses. Moreover, the robust stability (retaining functionality for four weeks) of Au@Pt nanozymes further enhanced their practical utility.
Conclusion: This novel technology demonstrates high specificity for target DNA and achieved perfect agreement with RT-PCR in clinical validation. Its combination of operational simplicity, requiring no instrumentation, and remarkable stability positions it as a highly promising tool for diagnosing infectious diseases.
Keywords: nanozyme, core/shell nanostructures, nucleic acid detection, varicella-zoster virus, diagnosis