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已发表论文
林义平膝关节周围针刺疗法治疗膝骨关节炎的疗效及潜在机制
Authors Huang L, Deng X, Jin Y, Li J, Wu X, Xiong Y, Guo S, Yan S, Lu S, Chen D
Received 4 July 2025
Accepted for publication 21 October 2025
Published 7 November 2025 Volume 2025:18 Pages 5891—5906
DOI https://doi.org/10.2147/JPR.S547968
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 2
Editor who approved publication: Dr Houman Danesh
Lijun Huang,1 Xingyou Deng,1 Yanling Jin,1 Jing Li,1 Xiangnong Wu,1 Youlong Xiong,1 Shaofeng Guo,2 Shaoyue Yan,3 Shengdi Lu,1 Dajun Chen4
1Yunnan Provincial Hospital of Traditional Chinese Medicine, Kunming, Yunnan, People’s Republic of China; 2Yiliang County North Ancient Town Central Hospital, Kunming, Yunnan, People’s Republic of China; 3Ma Zhan Township Health Center, Tengchong, Yunnan, People’s Republic of China; 4Yanshan County Hospital of Traditional Chinese Medicine Benge Branch, Yunnan, People’s Republic of China
Correspondence: Xingyou Deng, Yunnan Provincial Hospital of Traditional Chinese Medicine, Kunming, Yunnan, 650051, People’s Republic of China, Email 417772851@qq.com
Background: Degenerative knee osteoarthritis (KOA) may lead to disability in adults. Current treatment modalities for KOA often involve significant side effects and high costs. Yi-Ping Lin Circum-knee Acupuncture (CKA-L) emerged as a cost-effective and nearly side-effect-free alternative. However, its efficacy and the underlying mechanisms remain unclear, which raised our concern.
Methods: Female SD rats were randomly divided into 4 groups: Sham, Model, CKA-L treatment (Treatment), and positive control (Piroxicam). The Arthritis Index (AI) and body weights were recorded. ELISA was used to quantify levels of IL-1β and TNF-α. X-ray, HE, and Toluidine blue staining were employed to assess cartilage regeneration. Transcriptome sequencing was performed to identify differentially expressed genes (DEGs). Bioinformatics methods were employed to perform functional enrichment and protein-protein interaction (PPI) analysis on these DEGs. RT-qPCR and Western blot were utilized to determine the hub genes.
Results: Both CKA-L and piroxicam effectively alleviated KOA, albeit with slower response times for CKA-L (4 weeks in CKA-L group vs 3 weeks in piroxicam group). ELISA results demonstrated significant reductions in pro-inflammatory cytokines, with a pronounced decline in TNF-α in the Treatment group (p< 0.01). CKA-L markedly increased chondrocyte populations and narrowed the knee joint gap. Transcriptome analysis identified 3363 DEGs. Functional enrichment analyses highlighted significant associations with DNA repair, inhibition of cysteine-type endopeptidases, and cell-cycle regulation. The PPI analysis of DEGs designated five hub genes: Bgn, Ctsb, Lum, Serpine1, and Tnfaip6, which demonstrated consistent transcriptional and translational expression levels.
Conclusion: CKA-L promotes chondrocyte proliferation and provides effective treatment for KOA, with the advantages of low price, low side effects, and convenient operation.
Keywords: degenerative knee osteoarthritis, acupuncture, transcriptome sequencing, Osteoarthritis, traditional Chinese medicine
1Yunnan Provincial Hospital of Traditional Chinese Medicine, Kunming, Yunnan, People’s Republic of China; 2Yiliang County North Ancient Town Central Hospital, Kunming, Yunnan, People’s Republic of China; 3Ma Zhan Township Health Center, Tengchong, Yunnan, People’s Republic of China; 4Yanshan County Hospital of Traditional Chinese Medicine Benge Branch, Yunnan, People’s Republic of China
Correspondence: Xingyou Deng, Yunnan Provincial Hospital of Traditional Chinese Medicine, Kunming, Yunnan, 650051, People’s Republic of China, Email 417772851@qq.com
Background: Degenerative knee osteoarthritis (KOA) may lead to disability in adults. Current treatment modalities for KOA often involve significant side effects and high costs. Yi-Ping Lin Circum-knee Acupuncture (CKA-L) emerged as a cost-effective and nearly side-effect-free alternative. However, its efficacy and the underlying mechanisms remain unclear, which raised our concern.
Methods: Female SD rats were randomly divided into 4 groups: Sham, Model, CKA-L treatment (Treatment), and positive control (Piroxicam). The Arthritis Index (AI) and body weights were recorded. ELISA was used to quantify levels of IL-1β and TNF-α. X-ray, HE, and Toluidine blue staining were employed to assess cartilage regeneration. Transcriptome sequencing was performed to identify differentially expressed genes (DEGs). Bioinformatics methods were employed to perform functional enrichment and protein-protein interaction (PPI) analysis on these DEGs. RT-qPCR and Western blot were utilized to determine the hub genes.
Results: Both CKA-L and piroxicam effectively alleviated KOA, albeit with slower response times for CKA-L (4 weeks in CKA-L group vs 3 weeks in piroxicam group). ELISA results demonstrated significant reductions in pro-inflammatory cytokines, with a pronounced decline in TNF-α in the Treatment group (p< 0.01). CKA-L markedly increased chondrocyte populations and narrowed the knee joint gap. Transcriptome analysis identified 3363 DEGs. Functional enrichment analyses highlighted significant associations with DNA repair, inhibition of cysteine-type endopeptidases, and cell-cycle regulation. The PPI analysis of DEGs designated five hub genes: Bgn, Ctsb, Lum, Serpine1, and Tnfaip6, which demonstrated consistent transcriptional and translational expression levels.
Conclusion: CKA-L promotes chondrocyte proliferation and provides effective treatment for KOA, with the advantages of low price, low side effects, and convenient operation.
Keywords: degenerative knee osteoarthritis, acupuncture, transcriptome sequencing, Osteoarthritis, traditional Chinese medicine