109906
论文已发表
注册即可获取德孚的最新动态
IF 收录期刊
- 3.4 Breast Cancer (Dove Med Press)
- 3.2 Clin Epidemiol
- 2.6 Cancer Manag Res
- 2.9 Infect Drug Resist
- 3.7 Clin Interv Aging
- 5.1 Drug Des Dev Ther
- 3.1 Int J Chronic Obstr
- 6.6 Int J Nanomed
- 2.6 Int J Women's Health
- 2.9 Neuropsych Dis Treat
- 2.8 OncoTargets Ther
- 2.0 Patient Prefer Adher
- 2.2 Ther Clin Risk Manag
- 2.5 J Pain Res
- 3.0 Diabet Metab Synd Ob
- 3.2 Psychol Res Behav Ma
- 3.4 Nat Sci Sleep
- 1.8 Pharmgenomics Pers Med
- 2.0 Risk Manag Healthc Policy
- 4.1 J Inflamm Res
- 2.0 Int J Gen Med
- 3.4 J Hepatocell Carcinoma
- 3.0 J Asthma Allergy
- 2.2 Clin Cosmet Investig Dermatol
- 2.4 J Multidiscip Healthc
已发表论文
骨髓间充质干细胞中阳离子脂质体介导的 Bcl-2 基因转染：一种治疗脊髓损伤的新型再生方法
Authors Yuan H, Feng X, Hidayat Ullah K , Chu B, Wang T, Zhou X, Gu J
Received 3 January 2025
Accepted for publication 12 November 2025
Published 19 November 2025 Volume 2025:19 Pages 10225—10242
DOI https://doi.org/10.2147/DDDT.S513105
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 2
Editor who approved publication: Dr Leonidas Panos
Haitao Yuan,1,* Xiaojun Feng,1,* Kamran Hidayat Ullah,2 Bo Chu,1 Tianqi Wang,1 Xin Zhou,1 Jun Gu1
1Department of Orthopedics, Xishan People’s Hospital Of Wuxi City, Wuxi, Jiangsu, 204105, People’s Republic of China; 2Department of Pharmacy, CECOS University of IT and Emerging Sciences, Peshawar, Pakistan
*These authors contributed equally to this work
Correspondence: Jun Gu, Email wxgujun@njmu.edu.cn
Objective: This study explored a novel therapy for spinal cord injury (SCI) using cationic liposomes to deliver the anti-apoptotic Bcl-2 gene into rat bone marrow mesenchymal stem cells (BMSCs), followed by transplantation into a rat SCI model to evaluate its potential in promoting neural regeneration and enhancing SCI therapy.
Methods: BMSCs were isolated and characterized for surface markers and differentiation potential, and transfected with Bcl-2 via cationic liposomes. A rat SCI model was established to assess the therapeutic effects of BMSCs and Bcl-2-BMSCs. Functional recovery was evaluated using the Basso, Beattie, and Bresnahan (BBB) scale and inclined plate test, while histopathology, Western blot, and real-time polymerase chain reaction (RT-PCR) analyses were performed to assess neural recovery. Nissl and myelin staining were used to evaluate neuronal and myelin recovery.
Results: Following successful characterization of BMSCs and the spherical cationic liposomes (105 ± 1.3 nm, 18.65 ± 1.37 mV zeta potential), optimal transfection conditions were identified. Bcl-2-transfected BMSCs expressed high levels of Bcl-2 with low toxicity. In addition, the SCI results showed significant improvements in the BBB and inclined plate scores of rats in the BMSCs and Bcl-2-BMSCs groups compared to the model group (p< 0.01). HE-stained samples demonstrated that the secretion of Bcl-2 and BMSCs plays a crucial role in SC repair. Western blot results revealed an upregulation of proteins associated with neural recovery (Bcl-2, β-tubulin3, MAP2, NF-200, MBP) and a downregulation of the glial scar-associated protein GFAP in both the BMSCs and Bcl-2-BMSCs groups. Furthermore, Nissl staining and myelin staining showed that both the BMSCs and Bcl-2-BMSCs groups exhibited enhanced neuronal survival, inhibition of demyelination, and significant myelin regeneration.
Conclusion: Transplantation of Bcl-2-transfected BMSCs represents a highly promising strategy that effectively promotes neural regeneration, inhibits scar formation, reduces demyelination, and enhances functional recovery, highlighting its potential for clinical translation.
Keywords: bone marrow stromal cells, Bcl-2, cationic liposomes, spinal cord injury
1Department of Orthopedics, Xishan People’s Hospital Of Wuxi City, Wuxi, Jiangsu, 204105, People’s Republic of China; 2Department of Pharmacy, CECOS University of IT and Emerging Sciences, Peshawar, Pakistan
*These authors contributed equally to this work
Correspondence: Jun Gu, Email wxgujun@njmu.edu.cn
Objective: This study explored a novel therapy for spinal cord injury (SCI) using cationic liposomes to deliver the anti-apoptotic Bcl-2 gene into rat bone marrow mesenchymal stem cells (BMSCs), followed by transplantation into a rat SCI model to evaluate its potential in promoting neural regeneration and enhancing SCI therapy.
Methods: BMSCs were isolated and characterized for surface markers and differentiation potential, and transfected with Bcl-2 via cationic liposomes. A rat SCI model was established to assess the therapeutic effects of BMSCs and Bcl-2-BMSCs. Functional recovery was evaluated using the Basso, Beattie, and Bresnahan (BBB) scale and inclined plate test, while histopathology, Western blot, and real-time polymerase chain reaction (RT-PCR) analyses were performed to assess neural recovery. Nissl and myelin staining were used to evaluate neuronal and myelin recovery.
Results: Following successful characterization of BMSCs and the spherical cationic liposomes (105 ± 1.3 nm, 18.65 ± 1.37 mV zeta potential), optimal transfection conditions were identified. Bcl-2-transfected BMSCs expressed high levels of Bcl-2 with low toxicity. In addition, the SCI results showed significant improvements in the BBB and inclined plate scores of rats in the BMSCs and Bcl-2-BMSCs groups compared to the model group (p< 0.01). HE-stained samples demonstrated that the secretion of Bcl-2 and BMSCs plays a crucial role in SC repair. Western blot results revealed an upregulation of proteins associated with neural recovery (Bcl-2, β-tubulin3, MAP2, NF-200, MBP) and a downregulation of the glial scar-associated protein GFAP in both the BMSCs and Bcl-2-BMSCs groups. Furthermore, Nissl staining and myelin staining showed that both the BMSCs and Bcl-2-BMSCs groups exhibited enhanced neuronal survival, inhibition of demyelination, and significant myelin regeneration.
Conclusion: Transplantation of Bcl-2-transfected BMSCs represents a highly promising strategy that effectively promotes neural regeneration, inhibits scar formation, reduces demyelination, and enhances functional recovery, highlighting its potential for clinical translation.
Keywords: bone marrow stromal cells, Bcl-2, cationic liposomes, spinal cord injury