109906
论文已发表
注册即可获取德孚的最新动态
IF 收录期刊
- 3.4 Breast Cancer (Dove Med Press)
- 3.2 Clin Epidemiol
- 2.6 Cancer Manag Res
- 2.9 Infect Drug Resist
- 3.7 Clin Interv Aging
- 5.1 Drug Des Dev Ther
- 3.1 Int J Chronic Obstr
- 6.6 Int J Nanomed
- 2.6 Int J Women's Health
- 2.9 Neuropsych Dis Treat
- 2.8 OncoTargets Ther
- 2.0 Patient Prefer Adher
- 2.2 Ther Clin Risk Manag
- 2.5 J Pain Res
- 3.0 Diabet Metab Synd Ob
- 3.2 Psychol Res Behav Ma
- 3.4 Nat Sci Sleep
- 1.8 Pharmgenomics Pers Med
- 2.0 Risk Manag Healthc Policy
- 4.1 J Inflamm Res
- 2.0 Int J Gen Med
- 3.4 J Hepatocell Carcinoma
- 3.0 J Asthma Allergy
- 2.2 Clin Cosmet Investig Dermatol
- 2.4 J Multidiscip Healthc
已发表论文
circXPNPEP3 作为糖尿病肾病生物标志物的诊断作用
Authors Liu M, Zhan Q, Zhu J, Zhao J, Min D
Received 24 May 2025
Accepted for publication 28 October 2025
Published 17 November 2025 Volume 2025:18 Pages 4287—4297
DOI https://doi.org/10.2147/DMSO.S542515
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 3
Editor who approved publication: Dr Rebecca Baqiyyah Conway
Miao Liu, Qiunan Zhan, Junjun Zhu, Junli Zhao, Danyan Min
Department of Nephrology, Shanghai University of Medicine & Health Sciences Affiliated Zhoupu Hospital, Shanghai Pudong New District Zhoupu Hospital, Shanghai, People’s Republic of China
Correspondence: Danyan Min, Email lizzy211@126.com
Background: Diabetic nephropathy (DN) is a common chronic microvascular complication of diabetes mellitus (DM). Circular RNAs (circRNAs) have emerged as ideal biomarkers for various diseases. Recent studies have shown that circXPNPEP3 is upregulated in high glucose‐induced human umbilical vein endothelial cells. In this study, we aimed to examine the expression levels of circXPNPEP3 in the serum of patients with DN and to evaluate its diagnostic potential for this condition.
Methods: The expression levels of circXPNPEP3 in DN tissues and serum were detected using quantitative real-time polymerase chain reaction (qRT-PCR). DN was confirmed by biopsy or clinically defined as a urine albumin/creatinine ratio (uACR) > 30 mg/g or an estimated glomerular filtration rate (eGFR) < 60 mL/min per 1.73 m2. The association between circXPNPEP3 expression levels and the clinical features of patients with DN was investigated. Using healthy individuals as controls, the diagnostic value of circXPNPEP3 for DN was evaluated by receiver operating characteristic (ROC) curve analysis. Finally, a potential RNA-interaction-network involving circXPNPEP3 was constructed using bioinformatics approaches.
Results: The expression levels of circXPNPEP3 were significantly upregulated in both DN tissues and serum compared to those in non-DN tissues and serum from healthy controls. The dysregulation of circXPNPEP3 was significantly correlated with albuminuria categories and glomerular filtration rate (GFR) categories. Serum expression of circXPNPEP3 distinguished patients with DN from controls, yielding an area under the ROC curve (AUC) of 0.8389, with a sensitivity of 70.59% and a specificity of 86.27%. Finally, hsa-miR-135b-5p, hsa-miR-135a-3p, and hsa-miR-1237-3p were predicted to be potential targets of circXPNPEP3 in DN, and a competing endogenous RNA (ceRNA) network involving circXPNPEP3 was constructed based on bioinformatic predictions.
Conclusion: CircXPNPEP3 is upregulated in DN tissues and serum. Its elevated expression in serum shows promise as a non-invasive diagnostic biomarker for DN.
Keywords: diabetic nephropathy, circular RNA, biomarker, serum
Department of Nephrology, Shanghai University of Medicine & Health Sciences Affiliated Zhoupu Hospital, Shanghai Pudong New District Zhoupu Hospital, Shanghai, People’s Republic of China
Correspondence: Danyan Min, Email lizzy211@126.com
Background: Diabetic nephropathy (DN) is a common chronic microvascular complication of diabetes mellitus (DM). Circular RNAs (circRNAs) have emerged as ideal biomarkers for various diseases. Recent studies have shown that circXPNPEP3 is upregulated in high glucose‐induced human umbilical vein endothelial cells. In this study, we aimed to examine the expression levels of circXPNPEP3 in the serum of patients with DN and to evaluate its diagnostic potential for this condition.
Methods: The expression levels of circXPNPEP3 in DN tissues and serum were detected using quantitative real-time polymerase chain reaction (qRT-PCR). DN was confirmed by biopsy or clinically defined as a urine albumin/creatinine ratio (uACR) > 30 mg/g or an estimated glomerular filtration rate (eGFR) < 60 mL/min per 1.73 m2. The association between circXPNPEP3 expression levels and the clinical features of patients with DN was investigated. Using healthy individuals as controls, the diagnostic value of circXPNPEP3 for DN was evaluated by receiver operating characteristic (ROC) curve analysis. Finally, a potential RNA-interaction-network involving circXPNPEP3 was constructed using bioinformatics approaches.
Results: The expression levels of circXPNPEP3 were significantly upregulated in both DN tissues and serum compared to those in non-DN tissues and serum from healthy controls. The dysregulation of circXPNPEP3 was significantly correlated with albuminuria categories and glomerular filtration rate (GFR) categories. Serum expression of circXPNPEP3 distinguished patients with DN from controls, yielding an area under the ROC curve (AUC) of 0.8389, with a sensitivity of 70.59% and a specificity of 86.27%. Finally, hsa-miR-135b-5p, hsa-miR-135a-3p, and hsa-miR-1237-3p were predicted to be potential targets of circXPNPEP3 in DN, and a competing endogenous RNA (ceRNA) network involving circXPNPEP3 was constructed based on bioinformatic predictions.
Conclusion: CircXPNPEP3 is upregulated in DN tissues and serum. Its elevated expression in serum shows promise as a non-invasive diagnostic biomarker for DN.
Keywords: diabetic nephropathy, circular RNA, biomarker, serum