111614
论文已发表
注册即可获取德孚的最新动态
IF 收录期刊
- 3.4 Breast Cancer (Dove Med Press)
- 3.2 Clin Epidemiol
- 2.6 Cancer Manag Res
- 2.9 Infect Drug Resist
- 3.7 Clin Interv Aging
- 5.1 Drug Des Dev Ther
- 3.1 Int J Chronic Obstr
- 6.6 Int J Nanomed
- 2.6 Int J Women's Health
- 2.9 Neuropsych Dis Treat
- 2.8 OncoTargets Ther
- 2.0 Patient Prefer Adher
- 2.2 Ther Clin Risk Manag
- 2.5 J Pain Res
- 3.0 Diabet Metab Synd Ob
- 3.2 Psychol Res Behav Ma
- 3.4 Nat Sci Sleep
- 1.8 Pharmgenomics Pers Med
- 2.0 Risk Manag Healthc Policy
- 4.1 J Inflamm Res
- 2.0 Int J Gen Med
- 3.4 J Hepatocell Carcinoma
- 3.0 J Asthma Allergy
- 2.2 Clin Cosmet Investig Dermatol
- 2.4 J Multidiscip Healthc
已发表论文
基于多组学分析与实验验证的中医对银屑病的辨证论治综合研究
Authors Li C , Chen K , Qiao Y, Wu P, Chen C , Zhou L, Yang Y , Zhang J , Liu N , Shen C, Zhang G, Wang D, Gu J
Received 16 July 2025
Accepted for publication 6 November 2025
Published 26 November 2025 Volume 2025:18 Pages 16449—16472
DOI https://doi.org/10.2147/JIR.S554324
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 4
Editor who approved publication: Dr Anish R. Maskey
Canzhe Li,1,* Kaihao Chen,2,* Yuanyuan Qiao,1 Peilin Wu,1 Chupeng Chen,1 Lvzhou Zhou,3 Yibing Yang,1 Jin Zhang,1 Na Liu,1 Chuanjun Shen,1 Guangxian Zhang,1 Dongmei Wang,2 Jiangyong Gu1
1Research Centre of Basic Integrative Medicine, School of Basic Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, 510006, People’s Republic of China; 2Dermatology Hospital, Southern Medical University, Guangzhou, Guangdong, 510091, People’s Republic of China; 3The Second Clinical Medical College of Guangzhou University of Chinese Medicine, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, 510006, People’s Republic of China
*These authors contributed equally to this work
Correspondence: Dongmei Wang, Dermatology Hospital, Southern Medical University, Guangzhou, Guangdong, People’s Republic of China, Email 307984063@smu.edu.cn Jiangyong Gu, Research Centre of Basic Integrative Medicine, School of Basic Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, People’s Republic of China, Email gujy@gzucm.edu.cn
Purpose: The molecular basis underlying the clinical differentiation in treating psoriasis with syndromes of blood-heat or blood-stasis in traditional Chinese medicine remains unclear. This study employs an integrated multi-omics approach to explore the similarities and differences in the pathogenic mechanisms of these two syndromes, and to elucidate the molecular foundations of Compound Qingdai Capsule and Yinxiekang Pian, which are representative formulas for syndrome-specific treatment, thereby providing insights into the scientific connotation of syndrome differentiation and treatment.
Methods: Multi-omics data were integrated with machine learning to identify genes differentiating the two syndromes of psoriasis. The therapeutic mechanisms were explored through network pharmacology and molecular docking. Finally, in vivo experiments involved constructing composite psoriasis models in SD rats by combining traditional Chinese medicine syndromes with imiquimod induction, and analyzing skin lesion tissues via qPCR and ELISA.
Results: After constructing the psoriasis pathway network and successfully establishing rat models, the research identified the characteristic genes of blood-heat syndrome psoriasis as GSK3B, MTOR and CDK4, and those of blood-stasis syndrome psoriasis as AKT1, TNF and STAT3. STAT1 was found to be a common characteristic gene of both syndromes, while LTBP1, PROS1, and ALDH16A1 were the differential genes for distinguishing the two syndromes. Finally, Compound Qingdai Capsule and Yinxiekang Pian were shown to reduce the mRNA and protein expressions of GSK3B, AKT1 and STAT1 to varying degrees.
Conclusion: GSK3B and AKT1 are differentially expressed in blood-heat syndrome and blood-stasis syndrome psoriasis respectively, while STAT1 is a common inflammatory mediator for both. Compound Qingdai Capsule and Yinxiekang Pian respectively provide a theoretical basis for traditional Chinese medicine syndrome differentiation and treatment of psoriasis by intervening in the same or different inflammatory targets and signaling pathways.
Keywords: blood-heat syndrome, blood-stasis syndrome, multi-omics analysis, psoriasis, syndrome differentiation and treatment
1Research Centre of Basic Integrative Medicine, School of Basic Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, 510006, People’s Republic of China; 2Dermatology Hospital, Southern Medical University, Guangzhou, Guangdong, 510091, People’s Republic of China; 3The Second Clinical Medical College of Guangzhou University of Chinese Medicine, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, 510006, People’s Republic of China
*These authors contributed equally to this work
Correspondence: Dongmei Wang, Dermatology Hospital, Southern Medical University, Guangzhou, Guangdong, People’s Republic of China, Email 307984063@smu.edu.cn Jiangyong Gu, Research Centre of Basic Integrative Medicine, School of Basic Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, People’s Republic of China, Email gujy@gzucm.edu.cn
Purpose: The molecular basis underlying the clinical differentiation in treating psoriasis with syndromes of blood-heat or blood-stasis in traditional Chinese medicine remains unclear. This study employs an integrated multi-omics approach to explore the similarities and differences in the pathogenic mechanisms of these two syndromes, and to elucidate the molecular foundations of Compound Qingdai Capsule and Yinxiekang Pian, which are representative formulas for syndrome-specific treatment, thereby providing insights into the scientific connotation of syndrome differentiation and treatment.
Methods: Multi-omics data were integrated with machine learning to identify genes differentiating the two syndromes of psoriasis. The therapeutic mechanisms were explored through network pharmacology and molecular docking. Finally, in vivo experiments involved constructing composite psoriasis models in SD rats by combining traditional Chinese medicine syndromes with imiquimod induction, and analyzing skin lesion tissues via qPCR and ELISA.
Results: After constructing the psoriasis pathway network and successfully establishing rat models, the research identified the characteristic genes of blood-heat syndrome psoriasis as GSK3B, MTOR and CDK4, and those of blood-stasis syndrome psoriasis as AKT1, TNF and STAT3. STAT1 was found to be a common characteristic gene of both syndromes, while LTBP1, PROS1, and ALDH16A1 were the differential genes for distinguishing the two syndromes. Finally, Compound Qingdai Capsule and Yinxiekang Pian were shown to reduce the mRNA and protein expressions of GSK3B, AKT1 and STAT1 to varying degrees.
Conclusion: GSK3B and AKT1 are differentially expressed in blood-heat syndrome and blood-stasis syndrome psoriasis respectively, while STAT1 is a common inflammatory mediator for both. Compound Qingdai Capsule and Yinxiekang Pian respectively provide a theoretical basis for traditional Chinese medicine syndrome differentiation and treatment of psoriasis by intervening in the same or different inflammatory targets and signaling pathways.
Keywords: blood-heat syndrome, blood-stasis syndrome, multi-omics analysis, psoriasis, syndrome differentiation and treatment