111614
论文已发表
注册即可获取德孚的最新动态
IF 收录期刊
- 3.4 Breast Cancer (Dove Med Press)
- 3.2 Clin Epidemiol
- 2.6 Cancer Manag Res
- 2.9 Infect Drug Resist
- 3.7 Clin Interv Aging
- 5.1 Drug Des Dev Ther
- 3.1 Int J Chronic Obstr
- 6.6 Int J Nanomed
- 2.6 Int J Women's Health
- 2.9 Neuropsych Dis Treat
- 2.8 OncoTargets Ther
- 2.0 Patient Prefer Adher
- 2.2 Ther Clin Risk Manag
- 2.5 J Pain Res
- 3.0 Diabet Metab Synd Ob
- 3.2 Psychol Res Behav Ma
- 3.4 Nat Sci Sleep
- 1.8 Pharmgenomics Pers Med
- 2.0 Risk Manag Healthc Policy
- 4.1 J Inflamm Res
- 2.0 Int J Gen Med
- 3.4 J Hepatocell Carcinoma
- 3.0 J Asthma Allergy
- 2.2 Clin Cosmet Investig Dermatol
- 2.4 J Multidiscip Healthc
已发表论文
肠道定植的耐碳青霉烯类肠杆菌科细菌释放的外膜囊泡可降解抗生素并促进细菌存活
Authors Li P, Lin Y, Sun X, Huang J, Huang D, Xu Y
Received 14 August 2025
Accepted for publication 29 November 2025
Published 10 December 2025 Volume 2025:18 Pages 6509—6519
DOI https://doi.org/10.2147/IDR.S557028
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 3
Editor who approved publication: Dr Hemant Joshi
Peifen Li,* Yingying Lin,* Xihuan Sun,* Jiaming Huang, Donghong Huang, Yujin Xu
Clinical Microbiology Laboratory, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian, People’s Republic of China
*These authors contributed equally to this work
Correspondence: Yujin Xu, Clinical Microbiology Laboratory, The Second Affiliated Hospital of Fujian Medical University, Donghai Street No. 950, Quanzhou, Fujian, 362000, People’s Republic of China, Tel +86-18158090986, Fax +0595-22770258, Email xyj815@fjmu.edu.cn
Purpose: Gut colonization of carbapenem-resistant Enterobacterales (CRE) poses a significant risk for systemic infections, but the mechanisms driving resistance dissemination are poorly understood. This study aimed to investigate whether outer membrane vesicles (OMVs) secreted by gut-colonized CRE can enter the human circulatory system and mediate extracellular antibiotic resistance through functional carbapenemases and resistance genes.
Patients and Methods: We conducted comparative proteomic analyses of OMVs isolated from parental CRE strains and patient plasma samples. Antibiotic degradation assays were performed to evaluate OMV-mediated hydrolysis of imipenem and meropenem. In vitro experiments assessed the protective effects of OMVs on carbapenem-susceptible Escherichia coli and Pseudomonas aeruginosa. Additionally, a Galleria mellonella infection model was used to examine OMV-mediated bacterial survival under carbapenem pressure.
Results: Plasma-derived OMVs exhibited proteomic profiles similar to bacterial OMVs, including carbapenemase components, and demonstrated comparable antibiotic-degrading activity. These OMVs hydrolyzed 60– 75% of imipenem and meropenem within 24 hours, protecting susceptible bacteria from growth inhibition in vitro. Although no horizontal gene transfer was observed, OMVs enhanced Klebsiella pneumoniae survival under carbapenem pressure in the G. mellonella model, increasing larval survival rates by 25%.
Conclusion: Our findings reveal a novel OMV-mediated extracellular resistance mechanism that operates independently of genetic transfer, promoting bacterial persistence in the bloodstream. This study provides key insights into the role of OMVs in clinical treatment failure and identifies potential therapeutic targets to combat antibiotic resistance dissemination.
Keywords: outer membrane vesicles, carbapenem-resistant enterobacterales, CRE, carbapenemase, antibiotic degradation, intestinal colonization
Clinical Microbiology Laboratory, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian, People’s Republic of China
*These authors contributed equally to this work
Correspondence: Yujin Xu, Clinical Microbiology Laboratory, The Second Affiliated Hospital of Fujian Medical University, Donghai Street No. 950, Quanzhou, Fujian, 362000, People’s Republic of China, Tel +86-18158090986, Fax +0595-22770258, Email xyj815@fjmu.edu.cn
Purpose: Gut colonization of carbapenem-resistant Enterobacterales (CRE) poses a significant risk for systemic infections, but the mechanisms driving resistance dissemination are poorly understood. This study aimed to investigate whether outer membrane vesicles (OMVs) secreted by gut-colonized CRE can enter the human circulatory system and mediate extracellular antibiotic resistance through functional carbapenemases and resistance genes.
Patients and Methods: We conducted comparative proteomic analyses of OMVs isolated from parental CRE strains and patient plasma samples. Antibiotic degradation assays were performed to evaluate OMV-mediated hydrolysis of imipenem and meropenem. In vitro experiments assessed the protective effects of OMVs on carbapenem-susceptible Escherichia coli and Pseudomonas aeruginosa. Additionally, a Galleria mellonella infection model was used to examine OMV-mediated bacterial survival under carbapenem pressure.
Results: Plasma-derived OMVs exhibited proteomic profiles similar to bacterial OMVs, including carbapenemase components, and demonstrated comparable antibiotic-degrading activity. These OMVs hydrolyzed 60– 75% of imipenem and meropenem within 24 hours, protecting susceptible bacteria from growth inhibition in vitro. Although no horizontal gene transfer was observed, OMVs enhanced Klebsiella pneumoniae survival under carbapenem pressure in the G. mellonella model, increasing larval survival rates by 25%.
Conclusion: Our findings reveal a novel OMV-mediated extracellular resistance mechanism that operates independently of genetic transfer, promoting bacterial persistence in the bloodstream. This study provides key insights into the role of OMVs in clinical treatment failure and identifies potential therapeutic targets to combat antibiotic resistance dissemination.
Keywords: outer membrane vesicles, carbapenem-resistant enterobacterales, CRE, carbapenemase, antibiotic degradation, intestinal colonization