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已发表论文
携带blaVIM-2基因的肠道定植琼滕假单胞菌菌株的基因组特征分析
Authors Zhu W, Zhang J, Liu R, Fang L, Liu Y, Zhang H
Received 16 July 2025
Accepted for publication 23 October 2025
Published 10 December 2025 Volume 2025:18 Pages 6501—6507
DOI https://doi.org/10.2147/IDR.S554163
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 2
Editor who approved publication: Dr Hazrat Bilal
Weidong Zhu,1,* Junli Zhang,2,* Ruishan Liu,3 Lei Fang,3 Yi Liu,3 Huanran Zhang4
1Zhuji Affiliated Hospital of Wenzhou Medical University, Zhuji, Zhejiang Province, People’s Republic of China; 2Shaoxing Joint Teaching Base, Zhejiang Chinese Medical University, Shaoxing, Zhejiang Province, People’s Republic of China; 3State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University, Hangzhou, Zhejiang, People’s Republic of China; 4Department of Emergency Medicine, The First Affiliated Hospital, Zhejiang University, Hangzhou, Zhejiang, People’s Republic of China
*These authors contributed equally to this work
Correspondence: Huanran Zhang, Email zhang_huanran@zju.edu.cn
Introduction: To characterize the genomic architecture of carbapenemase-producing Pseudomonas juntendi harboring blaVIM-2, elucidate genetic mechanisms underlying carbapenem resistance, and evaluate mobile genetic element (MGE)-mediated dissemination pathways using Oxford Nanopore and Illumina sequencing were combined for hybrid genome assembly approaches.
Methods: Hybrid Nanopore-Illumina whole-genome sequencing was applied on two P. juntendi isolates (L2353hy/L2891hy) recovered from distinct human fecal samples. L2353hy and L2891hy were identified as P. juntendi by ANI analysis. Comparative pangenomics identified resistance determinants and phylogenetic relationships, and SNP distances were calculated using SNP-dists. Plasmid profiles were verified using S1 nuclease pulsed-field gel electrophoresis (S1-PFGE).
Results: Both strains exhibited a multidrug resistance profile, comprising 13 antimicrobial resistance genes (ARGs), including blaVIM-2, blaOXA-246, and tet(A). Core genome phylogeny demonstrated clonal propagation of two VIM-producing P. juntendi strains. Notably, these two isolates were closely linked to P. juntendi yb_3 (a fish intestinal isolate; Wenzhou, China).
Conclusion: This study reports two clonally related P. juntendi strains harboring blaVIM-2 isolated from human fecal microbiota, expanding the genomic understanding of carbapenem-resistant P. juntendi. The close phylogenetic relationship between these human isolates and an animal-derived strain (P. juntendi yb_3) underscores bidirectional resistance gene flow at the human-animal interface. Our findings support a One Health-oriented surveillance approach to mitigate the dissemination of carbapenemase-producing pathogens.
Keywords: Pseudomonas juntendi, blaVIM-2, whole-genome sequencing, antimicrobial resistance
1Zhuji Affiliated Hospital of Wenzhou Medical University, Zhuji, Zhejiang Province, People’s Republic of China; 2Shaoxing Joint Teaching Base, Zhejiang Chinese Medical University, Shaoxing, Zhejiang Province, People’s Republic of China; 3State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University, Hangzhou, Zhejiang, People’s Republic of China; 4Department of Emergency Medicine, The First Affiliated Hospital, Zhejiang University, Hangzhou, Zhejiang, People’s Republic of China
*These authors contributed equally to this work
Correspondence: Huanran Zhang, Email zhang_huanran@zju.edu.cn
Introduction: To characterize the genomic architecture of carbapenemase-producing Pseudomonas juntendi harboring blaVIM-2, elucidate genetic mechanisms underlying carbapenem resistance, and evaluate mobile genetic element (MGE)-mediated dissemination pathways using Oxford Nanopore and Illumina sequencing were combined for hybrid genome assembly approaches.
Methods: Hybrid Nanopore-Illumina whole-genome sequencing was applied on two P. juntendi isolates (L2353hy/L2891hy) recovered from distinct human fecal samples. L2353hy and L2891hy were identified as P. juntendi by ANI analysis. Comparative pangenomics identified resistance determinants and phylogenetic relationships, and SNP distances were calculated using SNP-dists. Plasmid profiles were verified using S1 nuclease pulsed-field gel electrophoresis (S1-PFGE).
Results: Both strains exhibited a multidrug resistance profile, comprising 13 antimicrobial resistance genes (ARGs), including blaVIM-2, blaOXA-246, and tet(A). Core genome phylogeny demonstrated clonal propagation of two VIM-producing P. juntendi strains. Notably, these two isolates were closely linked to P. juntendi yb_3 (a fish intestinal isolate; Wenzhou, China).
Conclusion: This study reports two clonally related P. juntendi strains harboring blaVIM-2 isolated from human fecal microbiota, expanding the genomic understanding of carbapenem-resistant P. juntendi. The close phylogenetic relationship between these human isolates and an animal-derived strain (P. juntendi yb_3) underscores bidirectional resistance gene flow at the human-animal interface. Our findings support a One Health-oriented surveillance approach to mitigate the dissemination of carbapenemase-producing pathogens.
Keywords: Pseudomonas juntendi, blaVIM-2, whole-genome sequencing, antimicrobial resistance