Introduction and objective: Precisely and sensitively diagnosing diseases especially early and accurate tumor diagnosis in clinical magnetic resonance (MR) scanner is a highly demanding but challenging task. Gadolinium (Gd) chelate is the most common T ₁ magnetic resonance imaging (MRI) contrast agent at present. However, traditional Gd-chelates are suffering from low relaxivity, which hampers its application in clinical diagnosis. Currently, the development of nano-sized Gd based T ₁ contrast agent, such as incorporating gadolinium chelate into nanocarriers, is an attractive and feasible strategy to enhance the T ₁ contrast capacity of Gd chelate. The objective of this study is to improve the T ₁ contrast ability of Gd-chelate by synthesizing nanoparticles (NPs) for accurate and early diagnosis in clinical diseases.
Methods: Reverse microemulsion method was used to coat iron oxide (IO) with tunable silica shell and form cores of NPs IO@SiO₂ at step one, then Gd-chelate was loaded on the surface of silica-coated iron oxide NPs. Finally, Gd-based silica coating magnetite NPs IO@SiO₂-DTPA-Gd was developed and tested the ability to detect tumor cells on the cellular and in vivo level.
Results: The r ₁ value of IO@SiO₂-DTPA-Gd NPs with the silica shell thickness of 12 nm was about 33.6 mM⁻¹s⁻¹, which was approximately 6 times higher than Gd-DTPA, and based on its high T ₁ contrast ability, IO@SiO₂-DTPA-Gd NPs could effectively detect tumor cells on the cellular and in vivo level.
Conclusion: Our findings revealed the improvement of T ₁ relaxation was not only because of the increase of molecular tumbling time caused by the IO@SiO₂ nanocarrier but also the generated magnetic field caused by the IO core. This nanostructure with high T ₁ contrast ability may open a new approach to construct high-performance T ₁ contrast agent.
Keywords: gadolinium chelate, silica, iron oxide, nanoparticles, T ₁ relaxivity, tumbling time